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Test Code LAB458 ANCA, IFA (IBD AND AUTOIMMUNE HEPATITIS) NOT FOR VASCULITIS

Important Note

This test includes reporting of P-ANCA (perinuclear), C-ANCA (cytoplasmic) and A-ANCA (atypical ANCA) patterns. This test is subject to reflex testing, see Laboratory Reflex Testing Policy, you have the option to decline reflex testing if you believe it is not medically necessary. If ANCAIF is positive at the screening dilution, an Anti Neutrophil Cytoplasmic Antibody Titer (CPT: 86256) will be performed. If a pattern is reported as P-ANCA or C-ANCA, a Myeloperoxidase Antibody and  a Proteinase 3 Antibody will be sent to MML. Analytical Time allows for repeat testing and titer of potential positive specimens.  Positive results will not be reported until the endpoint titer is determined.

Specimen Information

Container Specimen Temperature Collect Vol Submit Vol Min Vol Stability
SST  Serum Refrigerate 2 mL 1 mL 0.5 mL 48 hours
SST Serum Frozen 2 mL 1 mL 0.5 mL 1 month

Serum should be separated from the clot within 4 hours of collection.  Store samples at room temperature no longer than 8 hours.  If the assay will not be completed within 8 hours, refrigerate the sample at 2-8C.  If the assay will not be completed within 48 hours, or for shipment of the sample, freeze at -20C or lower.

Grossly hemolyzed, lipemic or icteric serum should be rejected.

 

Test Schedule / Analytical Time / Test Priority

Monday – Friday/ 2 - 5 days / Not available STAT

Reference Range

Negative

CPT Code(s)

Description CPT Code
Anti Neutrophil Cytoplasmic Antibody 86036
Anti-Neutrophil Cytoplasmic Antibody Titer (if appropriate) 86037

 

Method

Immunofluorescence

Instrumentation

Werfen QUANTA-Lyser and NOVA View

Performing Location

University of Vermont Medical Center

Section

Immunology

Springfield Hospital Laboratory Test Catalog Additional Information:

Alternate name:  Cytoplasmic Neutrophil AB