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HelpTest Code LAB9913 FISH TESTING (FLUORESCENCE IN SITU HYBRIDIZATION)
Specimen Information
| Container | Specimen | Temp | Collect Vol | Submit Vol | Min Vol |
| Bone Marrow Transport Media (sterile) | Bone Marrow | Ambient | 3 mL | 3 mL | 0.5 mL |
| Sodium Heparin Tube | Bone Marrow | Ambient | 3 mL | 3 mL | 0.5 mL |
| Sodium Heparin Tube, Adult* | Whole Blood | Ambient | 6 mL | 6 mL | 3 mL |
| Sodium Heparin Tube, Pediatric* | Whole Blood | Ambient | 3 mL | 3 mL | 1 mL |
| Hanks Solution | Lymph Tissue/Node | Ambient | 1 cm3 | 1 cm3 | 0.5 cm3 |
*Samples should be kept at ambient temperature after collection and during transport. Containers are available from Laboratory Customer Service 847-5121.
Some FISH testing can be done on unstained fresh tissue slides (air-dried FNA smear or touch imprints). Call the Cytogenetics Laboratory at 847-3565.
Containers are available from Laboratory Customer service 847-5121, bone marrow transport media (RPMI) supply #032047, sodium heparin supply #031977, hanks solution supply #032048. Use Hematopoietic Neoplasm Lab Form, supply #032014.
Test Schedule / Analytical Time / Test Priority
Monday – Saturday / 7 days / Not available STAT
Method
Culture, Microscopy,Probe Hybridzation, and Interpretation
CPT Code(s)
| Description | CPT Code |
| Blood /Bone Marrow Culture, Neoplastic | 88237 |
| FISH DNA Probe | 88271 |
| FISH Intrp & Report Part B | 88291 |
| In Situ Hybridization, Interphase, BM or Blood: 100-300 cells | 88275 |
| In Situ Hybridization, Interphase, BM or Blood: 25-99 cells | 88274 |
| Blood Culture | 88230 |
| In Situ Hybridization, Metaphase: 10-30 cells | 88273 |
| In Situ Hybridization, Metaphase: 3-5 cells | 88272 |
Instrumentation
Manual Method
Section
Cytogenetics
Performing Location
University of Vermont Medical Center
Is the UVMMC lab NY State Certified to perform this testing? Yes/No
Yes
Test Note
FISH Testing (Fluorescence In-Situ Hybridization):
- Fluorescent probes can be used to characterize chromosome abnormalities associated with specific hematologic diseases.
- Reporting time is 7 days.
- FISH testing can be done on the same sample submitted for chromosome evaluation or it can be ordered alone.
Some FISH testing can be done on unstained fresh tissue slides (air-dried FNA smear or touch imprints). Call the Cytogenetics Laboratory at 847-3565.
Hematologic (Neoplasia) Oncology probes available at UVMMC:
t(8;14) MYC/IGH, Burkitt’s Lymphoma
t(8;21) RUNX1/RUNX1T1, Acute Myeloid Leukemia (AML)
t(9;22) BCR/ABL, Chronic Myelogenous Leukemia (CML)
t(11;14) CCND1/IGH, Mantle Cell Lymphoma
t(12;21) ETV6/RUNX1, Acute Lymphoblastic Leukemia (ALL)
t(14;18) BCL2/IGH, Follicular Lymphoma
t(15;17) PML/RARA, Acute Promyelocytic Leukemia (APL)
CBFB Rearrangement inv (16), AML with eosinophils
MLL Rearrangement 11q23 AML, ALL or MDS
MYC Rearrangement 8q24, Burkitt’s Lymphoma
BCL2 Rearrangement 18q21 Large B Cell Lymphoma
BCL6 Rearrangement 3q27 Large B Cell Lymphoma
CLL FISH Panel, Chronic Lymphocytic Leukemia
Congenital Syndrome probes available at UVMMC:
DiGeorge Syndrome 22q11.2 deletion or duplication
Prader-Willi/ Angel man Syndrome 15q11.2 deletion or duplication
Smith-Magenis Syndrome 17p11.2 deletion or duplication
Williams Syndrome 7q11.23 deletion or duplication
CepX/SRY Xp11.1-q11.1 Yp11.3 enumeration or rearrangement of sex chromosomes
Send out FISH tests: require a separate sodium heparin tube (1-2mL)
Plasma Cell Proliferative Disorder, Myeloma FISH Panel