Person to be Tested

When to Collect

Type of Test

What to Collect

Symptomatic pregnant women, with possible Zika exposure

As soon as possible (≤12 weeks) after symptom onset

rRT-PCR assay*

1-2 mL of urine and 1-2 mL of serum

Symptomatic pregnant women, with recent travel to areas with active dengue transmission and risk of Zika

As soon as possible (≤12 weeks) after symptom onset

rRT-PCR assay* and dengue IgM only

1-2 mL of urine and 1 mL of serum

Pregnant women with possible Zika virus exposure and fetal prenatal ultrasound findings consistent with congenital Zika virus infection

As soon as congenital Zika virus infection is suspected

rRT-PCR assay* and Zika IgM

1-2 mL of maternal urine and 1 mL of maternal serum

Optional: amniocentesis specimen(s)