Test Code INSEC Stinging Insects Allergen Profile, Serum
Reporting Name
Stinging Insects Allergen ProfileUseful For
Establishing a diagnosis of an allergy to honeybee, yellow jacket, wasp, yellow faced hornet, and white-faced hornet venoms
Defining the allergen responsible for eliciting signs and symptoms
Identifying allergens:
-Responsible for allergic response and/or anaphylactic episode
-To confirm sensitization prior to beginning immunotherapy
-To investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
HBV | Honeybee Venom, IgE | Yes | Yes |
YJV | Yellow Jacket Venom, IgE | Yes | Yes |
WSPV | Wasp Venom, IgE | Yes | Yes |
YFHV | Yellow Faced Hornet Venom, IgE | Yes | Yes |
WFHV | White Faced Hornet Venom, IgE | Yes | Yes |
Performing Laboratory
Mayo Clinic Laboratories in RochesterSpecimen Type
SerumOrdering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.8 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 90 days |
Special Instructions
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86003 x 5
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
INSEC | Stinging Insects Allergen Profile | 94593-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
HBV | Honeybee Venom, IgE | 6844-5 |
WFHV | White Faced Hornet Venom, IgE | 6280-2 |
WSPV | Wasp Venom, IgE | 13176-3 |
YFHV | Yellow Faced Hornet Venom, IgE | 6288-5 |
YJV | Yellow Jacket Venom, IgE | 6740-5 |
Clinical Information
Clinical manifestations of immediate hypersensitivity (allergic) diseases are caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from IgE-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with an allergen.
In vitro serum testing for IgE antibodies provides an indication of the immune response to allergen that may be associated with allergic disease.
The allergens chosen for testing often depend upon the age of the patient, history of allergen exposure, season of the year, and clinical manifestations. In individuals predisposed to develop allergic disease, the sequence of sensitization and clinical manifestations proceed as follows: eczema and respiratory disease (rhinitis and bronchospasm) in infants and children younger than 5 years of age due to food sensitivity (milk, egg, soy, and wheat proteins) followed by respiratory disease (rhinitis and asthma) in older children and adults due to sensitivity to inhalant allergens (dust mite, mold, and pollen inhalants).
Cautions
Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity.
Some individuals with clinically insignificant sensitivity to allergens may have measurable levels of IgE antibodies in serum, and test results must be interpreted in the clinical context.
False-positive results for IgE antibodies may occur in patients with markedly elevated serum IgE (>2500 kU/L) due to nonspecific binding to allergen solid phases.
Report Available
Same day/1 day to 3 daysSpecimen Retention Time
14 daysReject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Forms
If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.
Testing Algorithm
This profile includes testing for honeybee, yellow jacket, wasp, yellow faced hornet, and white-faced hornet venoms.