Test Code LY27B HLA-B27, Blood
Reporting Name
HLA-B27, BUseful For
Assisting in the diagnostic process of ankylosing spondylitis, juvenile rheumatoid arthritis, and reactive arthritis
Performing Laboratory
Mayo Clinic Laboratories in RochesterSpecimen Type
Whole Blood EDTAOrdering Guidance
This test is best used alone if a particular disease, such as ankylosing spondylitis, is under consideration.
Orders received for both this test and 1LRR / Human Leukocyte Antigens (HLA) Class I Typing Low Resolution, Recipient, Blood or 1DIS / Human Leukocyte Antigens (HLA) A-B-C Disease Association Typing Low Resolution, Blood (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap.
Shipping Instructions
Specimen must arrive within 96 hours of collection.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Ambient | 4 days |
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86812
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
LY27B | HLA-B27, B | 26028-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
LY27 | HLA-B27 Result | 26028-1 |
B27C | Interpretation | 96625-9 |
Clinical Information
This major histocompatibility coded class I antigen is associated with ankylosing spondylitis, juvenile rheumatoid arthritis, and reactive arthritis. The mechanism of the association is not understood but probably is that of linkage disequilibrium.
There is an increased prevalence of human leukocyte antigen (HLA)-B27 in certain rheumatic diseases, particularly ankylosing spondylitis.
Studies have demonstrated that the HLA-B*27:06 allele, which is present in a small percentage of individuals of Asian ethnicity, may not be associated with ankylosing spondylitis.
Cautions
Extreme temperature changes during shipping may alter the specimen making it unacceptable for testing.
Report Available
2 to 6 daysSpecimen Retention Time
7 daysReject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Method Name
Flow Cytometry
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.