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Test Code RMCCSPUTUM Sputum Culture*

Methodology

Culture/Gram Stain

Performing Laboratory

Rutland Regional Medical Center Laboratory

Specimen Requirements

Submit only 1 of the following specimens: 
 
Specimen Type: Expectorated sputum

Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions:
1. Collect under direct supervision of nurse or physician.

2. Have patient remove dentures, if appropriate.

3. Instruct patient to take a deep breath, hold it momentarily, then cough deeply and vigorously into container, and collect expectorated material.
 

Specimen Type: Induced sputum

Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions:
1. Have patient remove dentures, if appropriate.

2. Instruct patient to brush his/her teeth and/or rinse mouth well with water.
3. With aid of nebulizer, have patient inhale approximately 25 mL of 3% to 10% sterile saline.

4. Collect expectorated material in container.
 

Specimen Transport Temperature

Ambient/Refrigerated NO/Frozen NO

Day(s) Performed

Daily

Analytical Time:

3 days

Test Classification and CPT Coding

CPT: 87070

         87205-Gram stain

LOINC: 6460-0

Order Code: RMCCSPUTUM

Result Code: RMCL800057

Result Name: Sputum Culture