Test Code RMCCURINE Urine Culture*
Methodology
Culture
Performing Laboratory
Rutland Regional Medical Center Laboratory
Specimen Requirements
Specimen must be refrigerated and arrive within 24 hours of collection. If transferred to a grey top culture tube, it is stable 48-72 hours refrigerated.
Specimen Type: Catheter, indwelling
Collection Container/Tube: Syringe
Submission Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions:
1. Avoid sending urine that has remained stagnant in catheter tubing for any length of time; do not send catheter bag urine; and avoid sending urine from catheters that have been in place longer than 5 to 9 days.
2. Clean catheter collection port with 70% alcohol.
3. Puncture with sterile needle and collect 5 mL to 10 mL of urine in sterile syringe.
4. Transfer >1 mL of urine into sterile container. Mix well.
5. Label container with patient’s name, identification number, date and time of collection, and type of specimen.
Specimen Type: Catheter, straight (in and out)
Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions:
1. Thoroughly cleanse urethral area with soap and water.
2. Rinse with gauze pads.
3. Aseptically insert catheter into bladder.
4. Allow approximately 15 mL to pass; then collect >1 mL of urine in sterile container. Mix well.
Additional Information: This collection is not recommended for routine urine culture because of potential contamination problems. Procedure may introduce urethral flora into bladder.
Specimen Type: Clean-catch, midstream
Container/Tube: Sterile container
Specimen Volume: >1 mL
Collection Instructions: Carefully collect midstream urine. Transport to laboratory as soon as possible or refrigerate immediately.
Specimen Transport Temperature
Refrigerated up to 24 hours.
Unpreserved urine is good for only 2 hours at room temperature, 24 hours if refrigerated.
Preserved urine is good for 48 hours at room temp or 2-8 Celsius.
Day(s) Performed
Daily
Analytical Time:
2 days
Test Classification and CPT Coding
CPT: 87088
LOINC: 630-4
Order Code: RMCCURINE
Result Code: RMCL800060
Result Name: Urine Culture