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HelpTest Code ROTA Rotavirus Antigen, Feces
Reporting Name
Rotavirus Ag, FUseful For
Investigation of patients with diarrhea, particularly infants, the elderly, and immunocompromised patients
Investigation of nosocomial diarrhea
Testing Algorithm
For information on other diagnostic tests that may be useful in the evaluation of a patient with diarrhea, see Laboratory Testing for Infectious Causes of Diarrhea.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
FecalSpecimen Required
Specimen Type: Fresh unpreserved feces
Supplies: Stool Collection Kit, Random (T635)
Container/Tube:
Preferred: Sterile fecal container
Acceptable: Swab
Specimen Volume: 5 to 10 grams
Collection Instructions: Place specimen in a tightly sealed plastic bag.
Specimen Minimum Volume
1 gram
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Frozen (preferred) | 7 days | |
| Refrigerated | 72 hours |
Special Instructions
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87425
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ROTA | Rotavirus Ag, F | 5880-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 24082 | Rotavirus Ag, F | 5880-0 |
Clinical Information
Rotavirus is a major cause of nonbacterial gastroenteritis, especially in infants and very young children (6 months-2 years of age) who have not received the rotavirus vaccine. Infection may be entirely asymptomatic or produce a spectrum of disease ranging from mild gastroenteritis to severe diarrhea and vomiting with dehydration. Infection usually begins acutely and lasts for 4 to 8 days. In temperate climates, rotaviral infections are seasonal; they peak in frequency during the winter months and are uncommon during the summer. Rotaviral gastroenteritis is, therefore, sometimes called "winter vomiting disease."
Infection is more likely to be symptomatic in preterm infants, immunosuppressed patients, and elderly individuals, especially those living in nursing homes or other confined quarters. In other children and adults, rotavirus infections are usually subclinical and may be associated with asymptomatic shedding of rotavirus in the feces.
Rapid and accurate detection of rotavirus antigens in fecal specimens may lead to better patient management, particularly in hospitalized or institutionalized patients.
Cautions
Fecal specimens should be collected as soon after onset of symptoms as possible.
Do not collect specimens in containers having media, preservatives, animal serum, or detergent as any of these may interfere with the assay.
A positive result does not preclude the presence of other pathogenic organisms. While the relationship between rotavirus and gastroenteritis is well established, coinfection with bacterial or parasitic pathogens is possible. If suspected, testing for other enteric pathogens should be performed in parallel with the rotavirus antigen test.
Results of the rotavirus antigen assay must be interpreted with caution. A negative result does not exclude the possibility of rotavirus infection, as too small a quantity of virus or inadequate or improper sampling may cause a false-negative result.
Report Available
1 to 3 daysSpecimen Retention Time
7 daysReject Due To
| Grossly bloody (containing no visible feces) Very mucoid feces Feces received in any preservative (example: Ecofix, formalin, PVA, C and S media) |
Reject |
Method Name
Enzyme Immunoassay (EIA)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Microbiology Test Request (T244)